10x chromium read length11/24/2023 DNA equivalent to about 150 copies of a genome does get distributed over about 1 million oil droplets that include beads. Thus, it allows individual long DNA molecules 10X Genomics Chromium Genome Linked-reads principle. The 10X Genomics technology generates individually barcoded sequencing libraries for hundreds of thousands of nanoliter volume oil droplets using up to 1.7 million different barcodes. Please see these slides for details on GemCode technology and some of the possible applications. Genotyping in repetitive regions of genomes is possible via an approach called “critical content rescue”. The applications currently supported by the 10X Genomics software are human haplotype phasing and structural variant detection (see this page for currently supported applications) as well as de novo genome assemblies (please see below). Just a few nanograms of sample ar e required as input for the library preparation. When analyzing high molecular weight DNA samples the linked read sequencing data delineate linkage information over distances of up to 150 kb. The sequencing libraries are generated with the Chromium controller instrument and reagents from 10X Genomics, followed by sequencing on our Illumina HiSeq sequencers. We are offering 10X Genomics Chromium Genome “linked read” library preps and sequencing. ![]() ![]() Please contact to reserve reagents for your project. As soon as these run out, we will discontinue the assay effective immediately. Update July 2020: We have ~16 remaining 10X Chromium Genome reactions left in our core. ![]() We are sad to see this very popular assay go. As of January 2020, 10X Genomics has announced that it is discontinuing production of the 10X Chromium Genome linked read reagents.
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